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KMID : 0043320170400080962
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Archives of Pharmacal Research
2017 Volume.40 No. 8 p.962 ~ p.971
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Determination of S-(-)-lansoprazole in dexlansoprazole preparation by capillary zone electrophoresis
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Chung Hyun-Kyu
Truong Quoc Ky
Mai Xuan Lan
Choi Yu-Sung
Kang Jong-Seong
Mar Woong-Chon
Kim Kyeong-Ho
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Abstract
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Capillary zone electrophoresis was successfully applied to the enantiomeric purity determination of dexlansoprazole using sulfobutyl ether-β-cyclodextrin and methyl-β-cyclodextrin as chiral selectors. Separations were carried out in a 50 μm, 64/56 cm fused-silica capillary. The optimized conditions included 90 mM phosphate buffer, pH 6.0, containing 30 mM sulfobutyl ether-β-cyclodextrin, 20 mM methyl-β-cyclodextrin as background electrolyte, an applied voltage of 25 kV and a temperature of 16 °C, detection was at 280 nm. The assay was validated for the S-(-)-lansoprazole in the range of 0.2-1.0%. The limit of detection was 0.07%, the limit of quantitation was 0.20%, relative to a total concentration of 4.0 mg mL-1. Intra-day precision varied between 1.72 and 2.07%. Relative standard deviations of inter-day precision ranged between 1.62 and 1.96% for peak area ratio. The assay was applied for the determination of the chiral purity of dexlansoprazole capsules. Recovery in capsules was ranged between 101.7 and 103.1%.
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KEYWORD
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Capillary electrophoresis, S-(-)-Lansoprazole, Enantiomeric impurity, Cyclodextrin derivatives, Dexlansoprazole preparation
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